Journal: Cytotechnology

Article Title: Perfluorooctanoic acid impaired glucose homeostasis through affecting adipose AKT pathway

doi: 10.1007/s10616-017-0164-6

Figure Lengend Snippet: PFOA induced changes of proteins in adipose tissue (immunostaining, scale bar = 100 μm). In immunohistochemistry and immunofluorescence assays, GLUT4-/p-AKT-positive cells were reduced and PTEN-labeled cells were increased in PFOA-treated adipose were observed in a dose-dependent manner. In addition, these positive cell counts showed statistical significance when compared to those in control. Scale bar: 100µm

Article Snippet: After washing for three times, the sections were incubated with anti-glucose transporter 4 (GLUT4) and p-AKT antibodies (1:200; Boster, Wuhan, China) overnight at 4 °C, followed by incubation with horseradish peroxidase (HRP) conjugated anti-rabbit secondary antibody (1:200; Boster, Wuhan, China) for 1 h at room temperature.

Techniques: Immunostaining, Immunohistochemistry, Immunofluorescence, Labeling, Control

Journal: World Journal of Biological Chemistry

Article Title: Current understanding of glucose transporter 4 expression and functional mechanisms

doi: 10.4331/wjbc.v11.i3.76

Figure Lengend Snippet: Recent studies of glucose transporter 4 expression and translocation in the skeletal muscle

Article Snippet: Immunoprecipitation, and Western blot. , Membrane fractions from skeletal muscle of male Wistar rats treated without or with insulin. Anti-GLUT-4 from Genzyme, Anti-GLUT-4 from Santa Cruz Biotechnology. , Crude membrane preparations and cytosolic fractions in samples of rats treated without or with insulin. , In vitro activation of PLD in crude membranes results in movement of GLUT4 to vesicles/microsomes. This GLUT4 translocation is blocked by the PLD inhibitor, neomycin, which also reduces insulin-stimulated glucose transport in rat soleus muscle. , [ ] .

Techniques: Expressing, Translocation Assay, Membrane, Control, Western Blot, Muscles, Clinical Proteomics, Real-time Polymerase Chain Reaction, Negative Control, Positive Control, Immunoprecipitation, Isolation, Transgenic Assay, Immunofluorescence, Microscopy, Electron Microscopy, Saline, Injection, In Vitro, Activation Assay, Labeling, Protease Inhibitor